Application of radioimmunological methods for checking the quality of class-specific antibodies against bovine and porcine immunoglobulins
Identifieur interne : 001642 ( Main/Exploration ); précédent : 001641; suivant : 001643Application of radioimmunological methods for checking the quality of class-specific antibodies against bovine and porcine immunoglobulins
Auteurs : L. Rodák [Tchécoslovaquie]Source :
- Veterinary Immunology and Immunopathology [ 0165-2427 ] ; 1984.
English descriptors
- Teeft :
- Antibody, Antiserum, Bovine, Bovine serum, Class specificity, Corresponding antigen, Double antibody sandwich, Immunoglobulin, Immunological studies, Normal serum, Porcine, Porcine immunoglobulins, Precipitation, Precipitation lines, Rabbit antiserum, Radioimmunological methods, Single precipitation line, Specific antibodies, Third incubations, Wells specificity, Wide range.
Abstract
Abstract: Class-specific antibodies against bovine IgG1, IgG2, IgM and IgA and porcine IgG, IgM and IgA immunoglobulins were prepared. Their class specificity was assessed by two radioimmunological methods, namely, radioimmunoelectrophoresis and double antibody sandwich radioimmunoassay. The methods are highly specific and sensitive and do not require the use of purified immunoglobulins, but can be performed with normal serum or colostrum. It was confirmed that antibodies found satisfactory in these tests were suitable for a wide range of use including radioimmunoassay and enzyme linked immunosorbent assay.
Url:
DOI: 10.1016/0165-2427(84)90006-0
Affiliations:
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Le document en format XML
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